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2 edition of Activation and inactivation of the sodium conductance in frog nerve and muscle fibres. found in the catalog.

Activation and inactivation of the sodium conductance in frog nerve and muscle fibres.

Carol Anne Collins

Activation and inactivation of the sodium conductance in frog nerve and muscle fibres.

by Carol Anne Collins

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  • 30 Currently reading

Published by Universityof East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.) - University of East Anglia, School of Biological Sciences, 1981.

ID Numbers
Open LibraryOL14512117M

6. The potassium currents of innervated rat muscle were similar to those of frog muscle in their voltage dependence of activation. 7. The time constant for inactivation of the sodium current, tau h, at ‐13 mV showed a temperature dependence measured between 10 and 20 degrees C . Claire O. Mal cot, Alain Duval, A macro cell-attached patch-clamp study of the properties of the Na current in the vicinity of the motor endplate region of frog single interosseal skeletal muscle fibres, Pfl gers Archiv European Journal of Physiology, /BF, , , (), ().

Collins, C. A., Arispe, N., and Rojas, E., , Slow Na+ conductance inactivation following modification of fast inactivation gating in frog muscle fibres glutaraldehyde on sodium channel activation and inactivation in frog nerve fiber and external sea anemone toxin ATX II on sodium channel inactivation of frog nerve fibres. (Figs. 1 and 2) and denervated (Fig. 3) tonic muscle fibres. It follows from the results that the slow calcium currents recorded from tonic muscle fibres of the frog (m. ileofibularis) resemble by both the activation and inactivation characteristics Ca currents recorded under similar conditions in phasic muscle fibres of the same muscle.

The steady-state and kinetic properties of Na+ and K+ currents of inner (white) and outer (red) muscles of zebrafish larvae 4–6 days post-fertilization (d.p.f.) are described. In inner muscle, the outward currents were half-activated at – mV and half-inactivated at– mV, and completely inactivated within ms of depolarization. The inward currents of inner fibres were half. (1) Action potentials and membrane currents were recorded in single myelinated rat nerve fibres at 20 and 37° C. Three experiments were also performed in single cat nerve fibres. (2) K currents were blocked by internal CsCl and external TEA. The steady state and kinetic parameters of Na activation and inactivation were determined at both temperatures.


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Activation and inactivation of the sodium conductance in frog nerve and muscle fibres by Carol Anne Collins Download PDF EPUB FB2

The steady-state and kinetic characteristics of the processes of activation and inactivation of the Na[+] permeability, P[Na], were measured in cut skeletal muscle fibres from Rana temporaria under voltage-clamp conditions.

The specific resistance, Cited by: Irreversible modification of sodium channel inactivation in toad myelinated nerve fibres by the oxidant chloramine‐T. Werner Ulbricht, Chloramine-T effect on sodium conductance of neuroblastoma cells as studied by whole-cell clamp and Effects of glutaraldehyde on sodium channel activation and inactivation in frog nerve fiber Cited by: Rojas E, Rudy B.

Destruction of the sodium conductance inactivation by a specific protease in perfused nerve fibres from Loligo. J Physiol. Nov; (2)– [PMC free article] Schwarz JR, Ulbricht W, Wagner HH. The rate of action of tetrodotoxin on myelinated nerve fibres of Xenopus laevis and Rana by: Activation and inactivation of the sodium conductance in frog nerve and muscle fibres.

Author: Collins, C. ISNI: Awarding Body: University of East Anglia Current Institution: University of East Anglia Date of Award: Availability of Full Text. Ca current inactivation was investigated in frog atrial muscle under voltage-clamp conditions.

To inhibit the outward currents, experiments were performed on Cs-loaded fibers and in 20 mM Cs (K-free) Ringer with 4-AP added. Inactivation, produced by a conditioning pulse, was measured by reducing the current during a subsequent test pulse.

The effect of calcium on contraction and conductance thresholds in frog skeletal muscle. The effect of sodium ions on the electrical activity of the giant axon of the squid. The effect of temperature on the sodium and potassium permeability changes in myelinated nerve fibres of Xenopus laevis.

Irreversible modification of sodium channel inactivation in toad myelinated nerve fibres by the oxidant chloramine-T. J Physiol. Jan; – [ PubMed ] Shrager P. Slow sodium inactivation in nerve after exposure to sulhydryl blocking reagents.

Voltage dependence of intramembrane charge movement and conductance activation of batrachotoxin-modified sodium channels in frog node of Ranvier.

J Gen Physiol. Jun; 81 (6)– [PMC free article] [Google Scholar] FRANKENHAEUSER B, HODGKIN AL. The after-effects of impulses in the giant nerve fibres of Loligo. Kinetic and pharmacological properties of the sodium channel of frog skeletal muscle.

gen. Physiol. 67, – Sodium inactivation in nerve fibres. Biophys. 8, – Hoyt, Molecular mechanism of sodium conductance changes in nerve. Abstract. The influence of temperature changes in the range of 25°C to −6°C on the time constants of Na activation (τm) and inactivation (τh) was studied in twitch muscle fibers and the node of Ranvier under voltage-clamp ius plots of τm and τh exhibit a change in activation enthalpy at temperatures below 10°C.

Cooling and subsequent heating induce a hystersis in the. Patlak JB, Ortiz M. Two modes of gating during late Na+ channel currents in frog sartorius muscle. J Gen Physiol. Feb; 87 (2)– [PMC free article] Rojas E, Rudy B. Destruction of the sodium conductance inactivation by a specific protease in perfused nerve fibres from Loligo.

J Physiol. Nov; (2)– Conti F, Neumcke B, Nonner W, Stämpfli R. Conductance fluctuations from the inactivation process of sodium channels in myelinated nerve fibres. J Physiol. Nov; – [PMC free article] Drouin H, Neumcke B. Specific and unspecific charges at the sodium channels of the nerve membrane.

Pflugers Arch. ; (3)– Inactivation of sodium conductance has been studied in squid axons with voltage clamp techniques and with the enzyme pronase which selectively destroys inactivation. Adult frog muscle appeared. The effects of glutaraldehyde on sodium channel gating were investigated in the membrane of the node of Ranvier in frog nerve fiber.

It was found that treating the membrane with glutaraldehyde slows the rate of inactivation, renders the inactivation curve considerably less steep, and leads to the appearance of a steady-state current component.

In this perspective I tell the story (albeit a clearly abridged version) of how our knowledge of ion conduction through ion channels has evolved from a purely electrical concept to a structural dynamics view of ions interacting with a membrane protein.

Our progress in this field has shown steady growth over the years but has also been interspersed with sudden jumps of discovery.

(a) Tubular sodium depletion In an attempt to demonstrate tubular sodium depletion during rapid repetitive stimu- lation, Bezanilla et al. () have examined the time course of tetanic tension in frog Contractile activation in skeletal muscle twitch fibers in solutions of varying sodium. Campbell DT.

Sodium channel gating currents in frog skeletal muscle. J Gen Physiol. Nov; 82 (5)– [PMC free article] Chiu SY, Mrose HE, Ritchie JM.

Anomalous temperature dependence of the sodium conductance in rabbit nerve compared with frog nerve. Nature. May 24; ()– Duggleby RG. Dubois JM, Schneider MF, Khodorov BI.

Voltage dependence of intramembrane charge movement and conductance activation of batrachotoxin-modified sodium channels in frog node of Ranvier. J Gen Physiol. Jun; 81 (6)– [PMC free article] FRANKENHAEUSER B, HODGKIN AL. The after-effects of impulses in the giant nerve fibres of Loligo.

Normally, after the voltage-gated increase in sodium ion permeability, there is a rapid inactivation of the sodium conductance. This inactivation process can be blocked by treatment of the intracellular domain of the sodium channel protein with proteases and by a monoclonal antibody directed against a segment of the protein connecting domains 3.

Mutations in the adult human skeletal muscle Na + channel a subunit cause the disease paramyotonia congenita. Two paramyotonia congenita mutations, RH and RC, substitute histidine and cysteine for arginine in the S4 segment of domain 4.

These mutations, expressed in a cell line, have only small effects on the activation of Na+ currents, but mutant channels inactivate more slowly with. H. Meves, Inactivation of the sodium permeability in squid giant nerve fibres, Progress in Biophysics and Molecular Biology, /(79), 33, (), ().

Crossref Wolfhard Almers, Gating currents and charge movements in excitable membranes, Reviews of Physiology, Biochemistry and Pharmacology, Vol   Currents through normal and aconitine-modified sodium channels in perfused neuroblastoma cells were measured under voltage-clamp conditions.

Aconitine.On the electrogenic sodium pump in mammalian non‐myelinated nerve fibres and its activation by various external cations Roberto De Col, Karl Messlinger, Richard W. Carr, Conduction velocity is regulated by sodium channel inactivation in T. Hashiguchi, A.L. Padjen, Evidence for a sodium-dependent potassium conductance in frog.